Figure 4 of Kawashima, Mol Vis 2009; 15:1162-1168.

Figure 4. Nucleostemin expression and cellular senescence in primary mouse corneal epithelial culture. Bmi1 expression was mostly overlapped with nucleostemin expression (A) as p63 in different location. It was noted that some large cells were also Bmi1-positive and nucleostemin-negative. A: Senescent cells display the increase of cell size, and the expression of senescence-associated Beta-galactosidase (SA-ß-gal) activity. Nucleostemin-positive cells were not stained by SA-ß-gal. B: The SA-ß-gal is not found in small compact cells (B). Bars indicate 100μm.