Figure 6. Time-course of cytokine release in HCECs exposed to PAF. The levels of various cytokines (IL-6, IL-8, and GM-CSF) were determined by specific ELISA assays when PAF (1 µM) was added to HCECs isolated from different donor eyes. Data are shown as mean±SEM from three or more experiments. For reference purposes, the basal levels of each cytokine measured were as follows (pg/ml): IL-6=151±33; IL-8=479±77; GM-CSF=44±19.