Figure 4. Immunohistochemical localization of Ogg1 in the adult mouse eye. Using an antibody raised against human 8-oxoguanine DNA glycosylase, immunostaining is detected in in neuroretina (A),ciliary body (D), cornea (E), and lens (F). The Ogg1 staining appears in brown, and sections were counterstained with methyl green solution. No signal was detected when the specific anti-Ogg1 antibody was omitted (G). Ogg1 protein was mainly present in the ganglion cell layer (GCL), in photoreceptor inner segments (IS), and in the outer (OPL) and inner plexiform layers (IPL). B and C show a high magnification of the inner and the outer portions of retina, respectively. Ogg1 protein was also detected in non-neuronal cells: corneal epithelium (Cep), pigmentary epithelium (PCE) and nonpigmentary ciliary epithelium (NPCE), lens epithelium (Le), lens transition zone (Tz), and lens fibers (Lf). Scale bar equals 50 μm in A, D,and G, 20 μm in B, C, and E, and 45 µm in F. Abbreviations: corneal stroma (Cs); inner nuclear layer (INL); outer nuclear layer (ONL); retinal pigment epithelium (RPE).