Figure 2. Ogg1 messenger in mouse retina, forebrain and cerebellum with associated densitometric analysis of the PCR bands. A shows relative amount of 8-oxoguanine glycosylase (Ogg1) mRNA in the mouse retina, forebrain (brain) and cerebellum (Cereb). To determine the expression of Ogg1 mRNA in adult C57BL/6J mouse neuroretinal cells (n=3), forebrain (n=3), and cereb (n=3), we performed semiquantitative RT–PCR using specific primers for mouse Ogg1 mRNA and cyclophilin A mRNA (Cyclo). Cyclo mRNA was used as an internal control. The 400 bp band corresponds to Ogg1 PCR products and the 311 bp band corresponds to cyclophilin A PCR products. B represents comparison of the Ogg1 mRNA expression in neuroretina, liver, and testis. Quantitative RT–PCR was performed to determine the relative levels of Ogg1 mRNA in adult C57BL/6 mouse neuroretina (n=5), liver (n=5), and testis (n=5), Cyclo mRNA was used as an internal standard for normalization. Significant, higher levels of Ogg1 mRNA expression were observed in testis as compared to those obtained in the liver and the neuroretina. Values are means±SEM.