Figure 2. A novel R12C mutation of CRYAA. Sequencing results showed a substitution of C to T in CRYAA (c.34C>T), which led to a R12C amino acid change. In the upper panel, the wild type (WT) CRYAA DNA segment (starting from the coding position 28) and its coding amino acid sequence are listed on the left, and the sequencing image is shown on the right. The mutant DNA and polypeptide sequences and the sequencing image were shown in the lower panel. The mutation is indicated in italic. The sequence change in image is indicated by arrows.