Table 2 of Ju, Mol Vis 2009; 15:120-134.

Table 2. Raw Ct values and relative expression levels for GAPDH and OPA1 in differentiated RGC-5 cells following elevated hydrostatic pressure.

Group
number
Time		 Passage
number
Conditions		 Ct values
GAPDH		 Ct values
OPA1
ΔCt
ΔΔCt
Relative expression
1 Day 2 34 Control 17.06 28.81 11.75 −0.57 Upregulation by 47.6%
      30 mmHg 18.82 30 11.18

  Day 3   Control 17.96 29.17 11.21 0.62 Down-regulation by 37.5%
      30 mmHg 17.85 29.68 11.83

2 Day 2 28 Control 16.82 28.36 11.54 −0.48 Upregulation by 36.5%
      30 mmHg 16.82 27.88 11.06

  Day 3   Control 16.94 27.87 10.93 0.68 Down-regulation by 37.7%
      30 mmHg 17.35 28.96 11.61

3 Day 2 30 Control 16.98 28.34 11.36 −0.27 Upregulation by 20.6%
      30 mmHg 16.84 27.93 11.09

  Day 3   Control 18.16 28.51 10.35 0.5 Down-regulation by 31.5%
      30 mmHg 18.51 29.36 10.85

This table showed that expression for GAPDH and OPA1 was not dependent with passage number and GAPDH did not change with elevated hydrostatic pressure. The negative ∆∆Ct represents upregulation of gene expression and the positive ∆∆Ct represents down-regulation of gene expression.

Ju, Mol Vis 2009; 15:120-134. http://www.molvis.org/molvis/v15/a12

©2009 Molecular Vision http://www.molvis.org/molvis/

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