Figure 4. Analyses of astrocytes subjected
to pressure for iso[4]LGE2–protein adduct formation. A:
ELISA analysis was performed with antibodies to iso[4]LGE2–protein
adduct and 10 µg of the control and pressure-treated astrocyte lysate
(described in greater detail in Methods). Following pressure treatment,
the culture medium was immediately replaced with fresh medium, and the
cells were incubated at 37 °C for 16 h at atmospheric pressure.
Immunocytochemical analysis with iso[4]LGE2–protein adduct
antibody was carried out for control cells (B) and for
astrocytes (C) that were subjected to 3 h of pressure at
100 mmHg and then subsequently subjected to a recovery period of
16 h.