Figure 2. Increased iso[4]LGE₂ modification of proteins in astrocytes from glaucomatous optic nerve head. A representative western blot analysis is shown of total protein lysates (25 µg) from astrocytes derived from the human optic nerve head. Age and gender of the donors (all Caucasian individuals) have been depicted. A was probed with rabbit polyclonal antibodies to iso[4]LGE₂–protein adduct, and B was re-probed with mouse monoclonal antibody to GAPDH.