Figure 4. Gene expression of marker proteins and membrane transport proteins in NRCE cells. A: Gene expression of marker proteins. NRCE cells stably express collagen type IV α2 chain (COL4A2) and vascular endothelial growth factor receptor 2 (FLK1), but not von Willebrand factor (vWF) or keratin 12. B: Gene expression of membrane transport proteins. NRCE cells express voltage-dependent anion channel 3 (VDAC3), voltage-dependent anion channel 2 (VDAC2), aquaporin 1 (AQP1), and Na⁺-K⁺ ATPase alpha 1 subunit (ATP1A1). P, NRCE cells in primary culture; S, NRCE cells at passage 191. β-actin was used as a loading control.