Figure 9. Effect of Robo4 on the migration of RF/6A and human RPE cells. The migratory activity of both cell lines was estimated based on the number of cells that had migrated through the filter of the chamber. A: Migrated cells of NS siRNA-treated RF/6A cells. B: Migrated cells of Robo4 siRNA-treated RF/6A cells. C: Migrated cells of NS siRNA-treated RPE cells. D: Migrated cells of Robo4 siRNA-treated RPE cells. E: The data of relative migrated cells in NS and Robo4 siRNA group cells. Values are the means±SD of at least three independent experiments. The results showed that the number of migrating cells in the Robo4 siRNA-treated group was less than in the control siRNA-treated group (E, p<0.01). Abbreviations: control siRNA treated cells (NS); Robo4 siRNA-treated RF/6A cells (RF/6A siRNA); Robo4 siRNA-treated RPE cells (RPE siRNA). The migrated cells of NS group was set to 100%.