Figure 8. Effects of Robo4 on the spreading of RF/6A and RPE cells. Cell spreading was quantified by measuring the ratio of the cytoplasm area to the nuclear area in cells in each field. A: Cell spreading of NS siRNA-treated RPE cells. B: Cell spreading of Robo4 siRNA-treated RPE cells. C: Cell spreading of NS siRNA-treated RF/6A cells. D: Cell spreading of Robo4 siRNA-treated RF/6A cells. E: The data of relative ratio of the cytoplasm area to the nuclear area in NS and Robo4 siRNA group cells. Values are the means±SD of at least three independent experiments. Asterisks denote values significantly different from those of cells treated with Robo4 siRNA compared to NS siRNA (E, p<0.01). Abbreviations: control siRNA-treated cells (NS); Robo4 siRNA-treated cells (R4 siRNA). The ratio of the NS group was set to 100%. Bar denote 100 μm.