Figure 2. Robo4 expression in FVMs from a 60-year-old patient with a 10 year history of diabetes. Immunofluorescent staining showed Robo4-positive (A, E), CD31-positive (B), and GFAP-positive (F) staining in FVMs. Cell nuclei were stained with DAPI (C,G). The colocalization of Robo4 and CD31 (D), and Robo4 and GFAP (H) are also shown. Bar graph denote 50 μm.