Figure 11. Effect of Robo4 on the apoptosis of RF/6A and RPE cells. A: Cell apoptosis of NS siRNA-treated RF/6A cells. B: Cell apoptosis of Robo4 siRNA-treated RF/6A cells. C: Cell apoptosis of NS siRNA-treated RPE cells. D: Cell apoptosis of Robo4 siRNA-treated RPE cells. E: The data of relative cell apoptosis in NS and Robo4 siRNA group cells. The normal living cells (bottom left quadrants) showed low annexin V and propidium iodide staining. The early apoptotic cells (bottom right quadrants) showed high annexin V staining but low propidium iodide staining. The late apoptotic cells (top right quadrants) showed high annexin V and propidium iodide staining. The percentages of cells in the quadrants are indicated within the quadrant of all panels. Representative results of three separate experiments are shown. Values are the means±SD of at least three independent experiments. Asterisks denote values signigicantly different from those of cells treated with Robo4 siRNA compared to NS siRNA and UT group (E, p<0.01).