Figure 11. Effect of Robo4 on the
apoptosis of RF/6A and RPE cells. A: Cell apoptosis of NS
siRNA-treated RF/6A cells. B: Cell apoptosis of Robo4
siRNA-treated RF/6A cells. C: Cell apoptosis of NS
siRNA-treated RPE cells. D: Cell apoptosis of Robo4
siRNA-treated RPE cells. E: The data of relative cell apoptosis
in NS and Robo4 siRNA group cells. The normal living cells
(bottom left quadrants) showed low annexin V and propidium iodide
staining. The early apoptotic cells (bottom right quadrants) showed
high annexin V staining but low propidium iodide staining. The late
apoptotic cells (top right quadrants) showed high annexin V and
propidium iodide staining. The percentages of cells in the quadrants
are indicated within the quadrant of all panels. Representative results
of three separate experiments are shown. Values are the means±SD of at
least three independent experiments. Asterisks denote values
signigicantly different from those of cells treated with Robo4 siRNA
compared to NS siRNA and UT group (E, p<0.01).