Figure 1. RT–PCR analysis of Robo4 in FVMs derived from proliferative diabetic retinopathy patients. After 35 cycles, 10 μl each sample was separated electrophoretically through a 1.5% Tris–acetate–EDTA agarose gel, and an expected product at 102 bp for Robo4 were stained with ethidium bromide. 1–6 was on behalf of 6 patients.