Table 1 of Mabuchi, Mol Vis 2009; 15:1045-1049. Table 1 of Mabuchi, Mol Vis 2009; 15:1045-1049.
Table 1. The primers used in this study.
Allele specific primer PCR method |
Annealing temperature |
Product size |
|---|---|---|
| 1st PCR | ||
| Forward Primer: 5’-TGGTCCTCTGACTGCTCTTT-3’ | 55 °C | 531 bp |
| Reverse Primer: 5’-AGGGTGTGATGGGATGGATAA-3’ | ||
| 2nd PCR for G allele genotyping | ||
| Forward Primer: 5’-ATGCCAGAGGCTGCTCGCCG-3’ | 65 °C | 398 bp |
| Reverse Primer: 5’-AGGGTGTGATGGGATGGATAA-3’ | ||
| 2nd PCR for C allele genotyping | ||
| Forward Primer: 5’-TGGTCCTCTGACTGCTCTTT-3’ | 62 °C | 172 bp |
| Reverse Primer: 5’-TGCTGGTGCAGGGGCCTCGG-3’ | ||
| Pyrosequencing analysis | ||
| PCR forward primer: 5’-AATTCCATGGGACTGACTTTCTGC-3’ | 60 °C | 370 bp |
| PCR reverse primer: biotin labeled 5’-Bio-GGGAAGGGACAGAAGATGACAG-3’ | ||
| Sequencing reverse primer: 5’-GGACAAGGGTTGGGC-3’ | ||