Table 1 of Mabuchi, Mol Vis 2009; 15:1045-1049.


Table 1. The primers used in this study.


Allele specific primer PCR method
Annealing
temperature
Product
size
1st PCR
Forward Primer: 5’-TGGTCCTCTGACTGCTCTTT-3’ 55 °C 531 bp
Reverse Primer: 5’-AGGGTGTGATGGGATGGATAA-3’

2nd PCR for G allele genotyping
Forward Primer: 5’-ATGCCAGAGGCTGCTCGCCG-3’ 65 °C 398 bp
Reverse Primer: 5’-AGGGTGTGATGGGATGGATAA-3’

2nd PCR for C allele genotyping
Forward Primer: 5’-TGGTCCTCTGACTGCTCTTT-3’ 62 °C 172 bp
Reverse Primer: 5’-TGCTGGTGCAGGGGCCTCGG-3’

Pyrosequencing analysis
PCR forward primer: 5’-AATTCCATGGGACTGACTTTCTGC-3’ 60 °C 370 bp
PCR reverse primer: biotin labeled 5’-Bio-GGGAAGGGACAGAAGATGACAG-3’
Sequencing reverse primer: 5’-GGACAAGGGTTGGGC-3’