Figure 2. Southern blot analysis to determine the copy number of the human MUC5AC-DTA transgene in human MUC5AC-DTA(+/−) transgenic mice. Arrow at the left indicates the 4.7 kb MUC5AC-DTA transgene construct in the control and the fragments liberated from the genomic DNA of transgenic mice by HindIII digestion. The copy number of the transgene varied from 1 to 10 among the nine founder lines. Transgenic mouse lines, C1, C2, C3, C4, and C5, had approximately one copy of the minigene inserted into the genome of transgenic mouse line. C6, C7, C8, and C9 had more than one copy of the minigene inserted in tandem.