Figure 7. Effect of cyclopamine on the development of corneal neovascularization in vivo. A, B: Cauterization in the central cornea induced neovascularization (NV) in the peripheral cornea (arrows) in the control group.
Such NV formation is seen to be suppressed in the cyclopamine-treated group (B) when compared with the control (A). C: The length of NV in the peripheral cornea from the limbus is shown. The NV length is significantly reduced in the cyclopamine
group as compared with the control (p<0.05). D, E: Nuclear translocation of Gli3 (arrows) is detected in the corneal epithelium and stromal cells of the peripheral cornea
in PBS-treated control group at day 7 (D) while it is not observed in a cornea treated with topical cyclopamine (E). F, G: Induction of Sonic hedgehog is also seen to be suppressed in a treatment group (F) when compared with the control group (G). Nuclear counterstaining was done with DAPI. Bar, 50 μm.