Figure 6. Effect of exogenous Sonic hedgehog on the development of corneal neovascularization in vivo. A-D: Implantation of a polymer pellet-containing recombinant Shh induced marked NV in the stroma from limbal vessels (B) as compared with control corneas (A), indicating that Shh has a NV-promoting effect in vivo like in vitro. Pellet containing cyclopamine reduced NV formation
as compared with control (C, D), indicating that endogenous Shh also has a promoting role in the development of corneal NV. Cyclopamine indeed counteracted
exogenous Shh-induced NV. B shows the length of the NV developed from limbus in each group of treatment (E).