Figure 5. Expression pattern of Sonic hedgehog and CD31 in alkali-burned corneas. On day 5 (A,B) and 20 (C,D), neovascularization in the limbal region was stained for Sonic hedgehog (Shh; FITC). The healing epithelium as well as the stroma were labeled for Shh (C). On day 20, CD31-labeled (Rhodamin) neovascularization was observed in the area of the corneal stroma stained for Shh (E,F). Bar, 50 μm.