Appendix 1 of Vergara, Mol Vis 2009; 15:1000-1013.

Appendix 1. Regenerated retina immunolabeled by Xen-1 but not by RPE-65.

To access the data, click or select the words “Appendix 1.” This will initiate the download of a compressed (pdf) archive that contains the file. Tadpole eyes were retinectomized, and an FGF-2-soaked bead (asterisk) was introduced in them. These eyes were collected at 0 (B, G), 10 (C, H), 20 (D, I), and 30 (E, J) days postsurgery and processed for immunohistochemistry. Labeling by Xen-1, a neural marker, was observed in the intact retina (A). Its expression was lost inside the eye at 0 and 10 days postsurgery (B and C), but was detected again in the regenerated retina at 20 and 30 days postoperation (D and E). Inset in A is a close up of the boxed area, showing that the ciliary marginal zone (CMZ) and the nonpigmented ciliary body (NPCB) were positive for Xen-1. Inset in E is a dark-field a close up of the 30-day regenerated retina. RPE-65 expression was only detected in the RPE and not in the retina or other pigmented eye tissues at any of the time points analyzed (F-J). Abbreviations: retinal pigmented epithelium (RPE); lens (L); retina (R); optic nerve (ON); pigmented ciliary body (PCB); regenerated retina (RR); cornea (C). Scale bar in J represents 100 μm and applies to all panels.