Figure 4. Cyt c in MsrA deficient mouse lenses shows evidence of oxidation and aggregation. A: SDS-PAGE and immunoblotting of cyt c (5 µg) following CNBr cleavage of total lens protein extract with a cyt c specific antibody. Lens protein extracted from wild-type and MsrA knockout mouse lenses with no HBO treatment. Lane 1 – wild type mouse lens, Lane 2 – MsrA knockout mouse lens, Lane 3 – cyt c standard. B: Colloidal blue staining of the SDS-PAGE gel shown as a control for protein loading. C: SDS-PAGE and immunoblotting of cyt c (5 µg) following CNBr cleavage of total lens protein extract with a cyt c specific antibody. Lens proteins extracted from wild-type and MsrA knockout mouse lenses following HBO treatment. Lane 1 – wild type mouse lens, Lane 2 – MsrA knockout mouse lens, Lane 3 – cyt c standard. D: Colloidal blue staining of the SDS-PAGE gel shown as a control for protein loading.