Figure 2. MsrA interacts with the components of the electron transport chain in human lens epithelial cells. A: SDS-PAGE and immunoblotting of HLE mitochondrial protein immunoprecipitated using an antibody to MsrA was probed with antibodies to specific subunits of complexes I-V (CI=NDUFS4 [18 kDa], CII=70 kDa subunit, CIII=core 1 subunit [48 kDa], CIV=cytochrome c oxidase subunit I [57 kDa], CV=ATP synthase subunit beta [56 kDa]). B: SDS-PAGE and immunoblotting of bovine liver tissue supernatant immunoprecipitated using antibodies to specific subunits of complexes I-V (listed above) was probed with an antibody to MsrA. C: SDS-PAGE and immunoblotting of HLE mitochondrial extracts immunoprecipitated using an MsrA-specific antibody and probed with a cyt c-specific (12 kDa) antibody. D: SDS-PAGE and immunoblotting of HLE mitochondrial extract immunoprecipitated using a cyt c-specific antibody and probed with an MsrA-specific antibody.