Table 1 of Yamada, Mol Vis 2009; 15:974-979. Table 1 of Yamada, Mol Vis 2009; 15:974-979.
Table 1. PCR primers used for sequencing exons of TGFBI.
Exon |
Primer |
Primer sequence |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|---|
| 2–9 | cDNA-F1 | 5'-CGCCAAGTCGCCCTACCAG-3' | 60 | 1205 |
| cDNA-R1 | 5'-TTGGAGGGGTTCCATCTTTG-3' | |||
| 9–17 | cDNA-F2 | 5'-CTCATCCCAGACTCAGCCAA-3' | 60 | 1075 |
| cDNA-R2 | 5'-CACATCTCATTATGGTGCGGC-3' | |||
| 1 | DNA-1F | 5'-CCGCTCGCAGCTTACTTAAC-3' | 60 | 362 |
| DNA-1R | 5'-AGCGCTCCAATGCTGCAAGGT-3' | |||
| 4 | DNA-4F | 5'-CGTCCTCTCCACCTGTAGAT-3' | 62 | 350 |
| DNA-4R | 5'-GACTCCCATTCATCATGCCC-3' | |||
| 11 | DNA-11F | 5'-CAGCCTTAATAACCCATCCCA-3' | 58 | 375 |
| DNA-11R | 5'-AATCCCCAAGGTAGAAGAAAG-3' | |||
| 12 | DNA-12F | 5'-AGGAAAATACCTCTCAGCGTGG-3' | 60 | 293 |
| DNA-12R | 5'-ATGTGCCAACTGTTTGCTGC-3' | |||
| 13 | DNA-13F | 5'-GGGAGTTCTTCATTTCAGGG-3' | 58 | 365 |
| DNA-13R | 5'-ATTACACTCAGAGATTCGGG-3' | |||
| 14 | DNA-14F | 5'-GCCTGGGCGACAAGATTGA-3' | 58 | 419 |
| DNA-14R | 5'-CCAACAGCTCCCAATTCAC-3' |