Table 2 of Li, Mol Vis 2009; 15:955-961. Table 2 of Li, Mol Vis 2009; 15:955-961.
Table 2. Details of the primers, enzymes, and temperature used in PCR-RFLP genotyping.
SNPs |
dbSNP ID |
Forward primer |
Reverse primer |
Tm (°C) |
Restriction enzyme |
|---|---|---|---|---|---|
| −169C/T (fcrl3_3) | rs7528684 | GGAAAATAATACA | GGCTTTAAAA | 56.9 | BsmFI |
| AATGTACAGATTA | AACGGTGGTAC | ||||
| −110A/G (fcrl3_4) | rs11264799 | CTCAATCCCGGT | CTCATAACAAC | 56.9 | PleI |
| AGTGATACA | TTATGTGAGA | ||||
| +358C/G (fcrl3_5) | rs945635 | TTATAGCCCATCTA | CCGGGATTGAGA | 60.3 | HaeIII |
| CTCACTCAGGATCA | TACAAACAGCATTT | ||||
| +1381A/G (fcrl3_6) | rs3761959 | TCCGACTTTTTCA | TGATAGCAGCACTA | 60.3 | MspI |
| GTCTCTAGGTTTT | GCTTGGACATTCA |