Figure 5 of Oka, Mol Vis 2008; 14:815-822.


Figure 5. Localization of filensin and phakinin in the 10-week-old rat lens. The localization of filensin in 10-week-old rat lenses was examined by phase-contrast microscopy (A-D) and fluorescence immunochemistry using antibodies directed against the filensin rod domain (E-H), filensin outer tail domain (I-L), and phakinin (M-P). (A, E, I, and M) shallow cortex of the Wistar lens; (B, F, J, and N) shallow cortex of the pre-cataract SCR lens; (C, G, K, and O) deep cortex of the Wistar lens; (D, H, L and P) deep cortex of the SCR lens. The anti-filensin rod domain antibody localized to the membrane lining regions in the shallow cortices of Wistar and pre-cataract SCR lenses (E and F) and to the central region of the cytoplasm in the deep cortex of the Wistar lens (G). The anti-filensin rod domain antibody exhibited a diffuse staining pattern in the deep cortex of the cataract SCR lens (H). The anti-filensin outer tail domain antibody localized to the membrane lining region of the shallow cortex of the Wistar (I) and pre-cataract SCR lens (J) as well as the deep cortex of the Wistar lens (K). This antibody exhibited a diffuse staining pattern in the deep cortex of the pre-cataract SCR lens. The localization of phakinin (M-P) was similar to that of the filensin rod domain (E-H). Scale bar, 10 µm.