Figure 4. Western blot analysis of filensin. Western blot analysis was performed using antibodies directed against the filensin rod domain (A), the inner tail domain (B), and the outer tail domain (C). Water-soluble fractions of 10-week-old Wistar (lanes 1), pre-cataract SCR (lanes 2), and normal SCR (lanes 3) lenses; water-insoluble and urea-soluble fractions of 10-week-old Wistar (lanes 4), pre-cataract SCR (lanes 5), and normal SCR (lanes 6) lenses. Arrowheads in B and C indicate the 36 kDa filensin peptide. The schematic representation of the filensin rod and tail domains (D) shows the predicted antigen sites (arrows) and cleavage sites (arrowheads).