Figure 2. Western blot analysis of filensin and phakinin in the 10-week-old rat lens. SDS–PAGE and western blot analysis of the cortical and nuclear regions of lenses from 10-week-old Wistar rats, pre-cataract SCRs, and normal SCRs using an anti-filensin antibody (A) and an anti-phakinin antibody (B) are shown. Ten micrograms of protein were loaded in each lane (Lane 1, cortex of Wistar lens; lane 2, cortex of pre-cataract SCR lens; lane 3, cortex of normal SCR lens; lane 4, nucleus of Wistar lens; lane 5, nucleus of pre-cataract SCR lens; lane 6, nucleus of normal SCR lens). The levels of filensin and phakinin in the nuclear regions were lower in 10-week-old lenses than in 6-week-old lenses. The 94 kDa form of filensin was absent from the cortex of the pre-cataract SCR lens. The 50 kDa form of filensin was decreased and the 38 kDa form was increased in the cortices of pre-cataract lenses as compared to Wistar lenses.