Figure 4. Comparison of soluble and insoluble protein from adult zebrafish lens. Both soluble (A) and insoluble (B) fractions were focused on pH 5–8 IPG strips before SDS–PAGE. Labels indicate examples of spots that are more abundant in one specific protein fraction (α-, βB1-, βB3-, and γN2-crystallin) or are equal in abundance in both fractions (γN1-crystallin). The white arrows on gel B show two α-crystallins that are preferentially found in the insoluble fraction. Many of the preferentially insoluble spots appear to be truncations.