Figure 7. Immunohistochemical analysis of the proteins for the regulatory subunits of PP2A-A and PP2A-B in the retina, lens epithelium,
lens fiber and cornea of 15.5-day-old mouse eye. The experimental procedures are described in the Methods section. The two
antibodies used for western blot analysis in
Figure 6 were also used here in immunohistochemical analysis. Note that both A and B subunits of PP-2A were strongly expressed in
the cornea. In the retina, the B subunit is homogenously expressed in both the neuro-retina layer and pigment retina layer.
In contrast, the expression of the regulatory A subunits is stronger in the pigment layer than in the neuro-retina layer.
Expression of both A and B subunits of PP-2A seems stronger in the fiber cells than in the lens epithelium. In addition, while
the A subunit appears in both the central and cortical fibers, B subunit is mostly distributed in the cortical fibers.
A: hematoxylin and eosin (HE) staining of the section cut from15.5-day-old mouse eye.
B: The section cut from the same embryonic mouse eye of
A was used for immunohistochemistry analysis with normal serum.
C: The section from the same embryonic mouse eye of
A was used for immunohistochemistry staining with anti-PP2A-Aα/β antibody. Note that PP2A-Aα/β expression was different in
the two layers of retina.
D: The section from the same embryonic mouse eye of
A was used for immunohistochemistry staining with anti-PP2A-B α/β/γ antibody. Note that PP2A-Bα/β/γ expression was homogenous
in both layers of retina.. Bar: 0.2 mm.