of Geller, Mol Vis 2008; 14:691-705.


Appendix 2. Additional evolutionarily conserved region fluorescence data, genomic fragment locations, and primer sequences.

To access the data, click or select the words “Appendix 2.” Additional evolutionarily conserved regions (ECR) from CD44 and vimentin (VIM) were further studied; 32 sequences were PCR amplified from C57BL/6J mouse and Sprague-Dawley rat genomic DNAs, cloned into the pFTMGW vector, and transfected into cultured rat Müller cells. The sequences were initially screened by fluorescence microscopy. Subsequently, eGFP positive constructs were analyzed by flow cytometry. The table shows the eGFP expression and the mean fluorescent intensity (MFI), with values normalized and expressed as fold change relative to pFTMGW alone. Restriction endonuclease is abbreviated REN. The “†” symbol identifies referenced in Geller et al. (2007) [22] as Vim409. The “‡” (nd) denotes flow cytometry was not performed on constructs showing eGFP expression qualitatively similar to vector (pFTMGW) alone. eGFP and MFI values are shown as fold change in expression relative to pFTMGW vector alone. An (R) denotes that gene is arranged in a “reversed” orientation (3′-5′) relative to the chromosome’s centromere. The “#” symbol represents that tested in combination with VIMR2 or CD44R2; Figure 6. The “^” symbol denotes cloned and tested in reverse orientation; Figure 6. The “@” denotes sequence includes the 5′ UTR, the first protein coding exon, and part of the first intron. “NM” numbers immediately below the gene acronym refer to RefSeq mRNA ID numbers.