Molecular Vision: Ponsioen, Mol Vis 2008; 14:652-660. Table 3

Table 3. Immunocytochemical analysis of human retinal Müller cells

Collagen	Cytoplasm staining		Extracellular staining		Cytoplasm aspect
Collagen	FBS	G5	FBS	G5	FBS	G5
Type I	++	+	n.d.	++	Granular	Granular
Type II	+	+	n.d.	+	Diffuse	Diffuse - granular
Type III	+	+	n.d.	n.d.	Diffuse	Diffuse
Type IV	++	++	n.d.	n.d.	Granular	Granular
Type V	++	+	Granules	++	Granular - fibrillar	Diffuse
Type VI	+	++	Granules - fibers	n.d.	Granular	Granular
Type VII	+	++	n.d.	n.d.	Diffuse	Granular
Type IX	++	++	n.d.	n.d.	Diffuse	Diffuse
Type XI	++	+	Small granules	++	Diffuse	Diffuse
Type XVII	-	-	n.d.	n.d.	-	-

Müller cells were fixed on glass chamber slides and stained with specific antibodies against types I-VII, IX, XI, and XVII collagen. LM confirmed the intracellular expression of all the above-mentioned collagens except type XVII collagen. The aspect of the collagen staining differed in aspect (diffuse, granular, or fibrillar) and intensity. In the table, the following symbols and abbreviations are used: strongly positive (++), positive (+), negative (-), not detected (n.d.), medium with 10% fetal bovine serum (FBS), and medium with 1% G5 (G5).