Molecular Vision: Ponsioen, Mol Vis 2008; 14:652-660. Table 2

Table 2. Overview of the western blot results

Collagen	Molecular weight of collagen bands in Müller cells	Moleculr weight of collagen bands in culture medium	Molecular weight of collagen bands after type VII collagenase
Type I	140 kDa	140 kDa	None
Type II	150 kDa	150 kDa	None
Type III	180 kDa	180 kDa	None
Type IV	130 and 210 kDa	130 and 210 kDa	75, 90, 110, and 250 kDa
Type V	260 kDa	260 kDa	None
Type VI	120 and 230 kDa	120 and 230 kDa	150 kDa
Type VII	270 kDa	270 kDa	130, 135, and 140 kDa
Type IX	200 kDa	150 and 200 kDa	None
Type XI	100, 150, and 200 kDa	150 kDa	None
Type XVII	None	None	None

By SDS–PAGE, collagen secretion into the medium was established for types I-VII, IX, and XI collagen. The specific collagen antibodies detected the collagen bands (in kDa) obtained before and after treatment with type VII collagenase. The results of both Müller cell extracts and medium with G5 are shown. The results after type VII collagenase concern Müller cell extracts and confirm the collagen nature of the bands as seen by WB. “None” indicates no collagen bands were detected.