Figure 5. Protein levels of DNA repair enzymes are decreased in aged retinal pigment epithelium and choroid. A: Immunoblots of the DNA repair enzymes, OGG1 and MYH, are shown. Actin was used as internal reference. Each group of three bands represents protein extracts from both retinal pigment epithelium (RPE) and choroid of three different animals. B: The differences in expression levels were determined by scanning gels and determining the integrated areas of the bands using Image-J software. Data are expressed as normalized ratios to actin. There were significant decreases in aged RPE and choroid of OGG1 (p=0.02, n=3) and MYH (p=0.001, n=3), compared to young RPE and choroid.