Figure 2. Effect of phaseolus vulgaris agglutinin on repair of wounds in monolayers of 92–1, Mel202, FM55P, and IGR-39 cells. A line was scratched with a plastic pipette tip through the confluent monolayer of cells maintained in serum-containing RPMI 1640 on a fibronectin-coated surface. The wounded cultures were allowed to heal for 24 h at 37 °C in the presence or absence of 25 μg/ml phaseolus vulgaris agglutinin (PHA-L) in serum-containing RPMI 1640. A: Panels show migration of cells in the presence or absence of PHAL after 24 h. B: The extent of wound closure was quantified by measurements of the width of the wound space for each case. For this value, the width was measured at twenty different locations in the wound and the mean value was compared to the width of the original closure (0 h). Values are means ± standard deviation of three separate experiments. Asterisk (*) indicates p<0.05.