Figure 7. E2F3a/E2F5/p130 triple transgenic lens histology and BrdU incorporation assays. A-C: At E15.5, when compared with a non-transgenic FVB lens (A), the triple transgenic lens showed a modest phenotype with some displaced fiber cell nuclei(C) in contrast to the many fiber cell nuclei seen in the E2F3a single transgenic lens (B). Abbreviations: le, lens epithelium; lf, lens fiber. D-F: The number of BrdU positive fiber cells (arrow head) in the triple transgenic lens (F) decreased by about 50% when compared to the E2F3a single transgenic lens (E). Scale bars=500 μm.