Figure 6. Expression of E2F3a/E2F5/p130 proteins at E15.5. Immunohistochemistry was used to assess expression of E2F3a, E2F5 and p130 proteins in non-transgenic FVB (A, B, E, F, I, J) and triple transgenic lenses (C, D, G, H, K, L). There was no positive staining for Myc-E2F3a (A) or p130 (I) in FVB lens fiber cells. By comparison, intensive green nuclear staining was seen in the triple transgenic lens fiber cells for all three proteins (C, G, K). Cell nuclei were visualized by DAPI staining (B, D, F, H, J, L). Scale bars=500 μm.