Figure 3. Characterization of E2F1/E2F5 bigenic eyes at E14.5. When compared with FVB lens (
A), the bigenic lens histology shows alterations in fiber cell elongation, and the presence of extra nuclei in the center of
the lens (
B), similar to the lens phenotype in the E2F1 single transgenic lens [
29]. There are no BrdU positive fiber cells (brown nuclear stain) in the FVB lens (
C). BrdU positive fiber cells were prevalent in the double transgenic lens (
D). Abbreviations: le, lens epithelium; lf, lens fiber. Scale bars=500 μm.