Figure 8. Olomoucine treatment significantly decreases MMP-2 expression in HCLE cells after scratch wounding. A: Immunoblotting of protein extracts of HCLE cells with MMP-2 antibody detected a single immunoreactive band of approximately 68 kDa. Lane 1 shows that cultured cells expressed MMP-2 before scratch wounding. Lane 2 illustrates that scratch wounding decreased MMP-2 expression detected 18 h afterward. Lane 3 also shows that scratch wounding followed by 18 h incubation with olomoucine further decreased MMP-2 expression. B: The results of immunoblotting experiments were quantified by densitometry (n=4). Statistical analysis demonstrated a significant decrease in MMP-2 expression (p<0.05) in scratch wounded cultures when compared to unwounded cultures and in scratch wounded cultures with olomoucine when compared to those without it (p<0.05). Graph represents mean ± SEM. Values are normalized to unwounded controls.