Figure 7. Olomoucine treatment significantly increases MMP-9 expression in HCLE cells after scratch wounding. A: Immunoblotting of protein extracts of HCLE cells with MMP-9 antibody detected a single immunoreactive band of approximately 92 kDa. Lane 1 shows that cultured cells expressed MMP-9 before scratch wounding. Lane 2 illustrates that scratch wounding increased MMP-9 expression detected 18 h afterward. Lane 3 shows that scratch wounding followed by 18 h incubation with olomoucine further increased MMP-9 expression. B: The results of immunoblotting experiments were quantified by densitometry (n=4). Statistical analysis demonstrated a significant increase in MMP-9 expression (p<0.05) in scratch wounded cultures when compared to unwounded cultures and in scratch wounded cultures with olomoucine when compared to those without it. Graph represents mean ± SEM. Values are normalized to unwounded controls.