Figure 5. Olomoucine treatment increased the localization of MMP-9 protein in epithelial cells at the wound edge. A: MMP-9 immunofluorescence is elevated at the wound edge in untreated control corneas (arrow). B: The immunostaining of MMP-9 at the wound edge (arrow) is further enhanced in olomoucine-treated corneas. C: Whole mounted corneas without any primary antibody showed no immunofluorescence. D: Paraffin sections of wounded control corneas showed MMP-9 immunofluorescence in all layers of the cornea at wound edge (arrow) and in basal epithelial cells distal to the wound (arrowhead). E: Paraffin section of olomoucine-treated corneas showed intense MMP-9 immunofluorescence in a wider band of cells at the wound edge. Immunofluorescence was seen in cells of all layers of the epithelium (arrow). F: No immunostaining was detected in the paraffin sections of corneas when primary MMP-9 antibody was omitted. Scale bar=100 μM.