Table 1 of Yen, Mol Vis 2008; 14:487-494.

Table 1. Oligonucleotide primer pairs for polymerase chain reaction.

Name Oligonucleotide Annealing temperature (°C) Region Product size (bp)
1F AGGGAGCGGCTGGCTGTC 63.5+DMSO Exon 1 302
1R GGCGGGTACCGTTTTCAGG
2F TCCACATGGATGCCTCTACA 60 Exon 2 459
2R TTCCCATGCAAATCTTCAAA
3F CTGGGATTACAGGCGTGAG 54 Exon 3 628
3R GCCTTGCCAAATGCTAAATC Exon 4
5F GGCTAAGCATGGCATCTTTC 64 Exon 5 460
5R CTTCCAAGACCAGGCAAAAC
6F CTCAAAATCCTGGCCTCAAG 64+DMSO Exon 6 575
6R TTCAATTTGCCAGGACATGA
7F ATGGCGAGATGAAACTGACC 62.2 Exon 7 404
7R ATTTGACCTCCGGTGACAAG
8F TTGGAGAATGTTCTGGAAAGC 60.9 Exon 8 485
8R CAGAAAGCACATTGCTTGGA
9F TTTGAAAACCCCTGATCCTTT 62.2 Exon 9 496
9R TTGCAGTGAGCTGAGATCGT
10F GGATTGATTCACCAGCCAGT 62.2 Exon 10 449
10R AAGTTCTCCAGTCCCCAACC
11F CCTTGGGGTTTGTTTAAAAGC 62.2 Exon 11 489
11R TCACCCCACGACCTACTAGC
12F GCTAGTAGGTCGTGGGGTGA 64 Exon 12 420
12R CGCAATAAAGCACATTACACA
13F TATGTTGCCCAGGCTTGTCT 65+DMSO Exon 13 518
13R AGATCCACTGAGCACTTTCCA
14F TGCTATCGGAATGTACCTGGA 60 Exon 14 506
14R TCACATGAAGTGTAAGTGAAGCAA
15F TGAACCTTGGCAGTGTAGTTTG 64 Exon 15 373
15R GATTCGGTGGGTAATGGATG
16F CCTGTGCTCATGTCCCACTA 59+DMSO Exon 16 430
16R CTCCCAAAGTGCTGGGATTA

Primers were used in the amplification of OPTN exons and were used in sequencing reactions. Primers located in introns were placed far enough away from the exon boundaries to allow visualization of the sequence of the splice site. In the table, “F” indicates the forward strand and “R” indicates the reverse strand. In additional, bp represents base pair and DMSO represents dimethylsulfoxide in this table.

Yen, Mol Vis 2008; 14:487-494. http://www.molvis.org/molvis/v14/a58

©2008 Molecular Vision http://www.molvis.org/molvis/

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