Figure 5. Hypoxia-inducible green fluorescent expression is specific to ARPE-19 cells transfected with AAV.HRSE.6xHRE.Rpe65.GFP. Hypoxia-inducible green fluorescent protein (GFP) expression is specific to RPE (ARPE-19) cells transfected with the AAV.HRSE.6xHRE.Rpe65.GFP vector. Cells were transfected with AAV.HRSE.6xHRE.Rpe65.GFP and cultured for seven days before forty hours of hypoxia (pO₂=1%). Hypoxia-inducible GFP expression is specific to only ARPE-19 cells and was not detected in transfected HT22 mouse hippocampal neurons, HEK-293 human embryonic kidney or C6 glioma cell lines cultured under similar conditions. Duplicate phase contrast images detail the morphology of the transfected cell lines in column one. In column three, representative images of control cultures infected with AAV.CMV.GFP express GFP constitutively during normoxic conditions.