Molecular Vision: Green-Church, Mol Vis 2008; 14:456-470. Table 2

Table 2. Summary of proteins observed by MudPIT after fractionation with ammonium sulfate.

Protein from capillary	Precipitant	Supernatant
Lactoferrin	X	X
Lipocalin 1	X	X
Poly Ig Receptor	X	X
Ig A1 Bur	X	X
Zn-alpha2-glycoprotein	X	X
Proline Rich 4	X	X
Cystatin S	X
Ig Alpha 1	X	X
Ig Lambda	X
Ig Alpha 2	X	X
Secretoglobin, family 2A Member 1	X	X
Protein Len, Bence-Jones	X
Prolactin Induced Protein	X	X
Protein Rei, Bence-Jones	X
Ig Kappa	X
Lysozyme	X	X
Lacritin	X	X
Basic proline rich protein	X
DMBT1	X
Ig J	X
Keratin 1	X	X
Lipophilin A	X	X
Haptoglobin	X
CTBP2 Protein	X
Transcoalbumin I	X
Keratin 9		X
Keratin 2a		X
Protein from Schirmer	Precipitant	Supernatant
Lactoferrin	X	X
Lipocalin 1	X	X
Poly Ig Receptor	X
Zn-alpha2-glycoprotein	X
Proline Rich 4	X	X
Ig A1 Bur	X	X
Prolactin Induced Protein	X	X
Ig Alpha 2	X	X
Ig Alpha 1	X
Secretoglobin, family 2A Member 1	X
Protein Len, Bence-Jones	X
Protein Rei, Bence-Jones	X
Lysozyme	X	X
Ig Kappa	X	X
Cystatin S	X	X
Lactritin	X	X
Basic proline rich protein	X
Ig J	X
Serum Albumin	X	X
DMBT1	X
HRPE773	X
Lipophilin A	X
Haptoglobin	X
Ig Gamma	X
Keratin 1	X	X
actin, beta	X	X
Keratin 9		X

Ammonium sulfate precipitation was used to fractionate and remove the high abundant proteins from less abundant proteins. This Table summarizes the proteins identified from the various fraction and collection methods. In both the capillary-collected tears and Schirmer-collected tears, the precipitant fraction contained the most proteins and the supernatant contained what are known to be the highly abundant proteins in the tear.