Table 1 of Achari, Mol Vis 2008; 14:443-455.

Table 1. Primers used in reverse transcription polymerase chain reaction experiments.

Gene Forward primer Reverse primer Tm (°C) bp Source
ER GTGTCTGTGATCTTGTCC CTCCATGATCAGGTCCAC 60 341 GenBank X73067
PR CCACAGTACAGCTTCGAGTC CCTGCATAGATCACATCTGG 60 431 GenBank M14547
Col-I GATGCGTTCCAGTTCGAGTA GGTCTTCCGGTGGTCTTGTA 55 312 Kao, W.W. Personal communication
Col-III TTATAAACCAACCTCTTCCT TATTATAGCACCATTGAGAC 55 255 Pelliniemi, L., Vuorio, E., Personal communication
Col-V GAGGAGAACCAGGAATAACC GCACCTTTCTCTCCGATGCC 55 215 Bluteau, G., Personal communication
Biglycan GATGGCCTGAAGCTCAA GGTTGTTGAAGAGGCTG 60 406 GenBank AF020290
Decorin TGTGGACAATGGTTCTCTGG CCACATTGCAGTTAGGTTCC 55/60 419 GenBank S76584
FGF TACAACTTCAAGCAGAAGAG CAGCTCTTAGCAGACATTGG 55 282 GenBank X04432, X04433
CTGF TTGTAGCTGATCAGTCTTTCCAC CAACTAAAAAGGTGCAAACATGTAA 60 119 GenBank NM_001901
IGF-1 GCATCCTGTCCTCCTCGCAT GTCTTGGGCATGTCGGTGTG 60 322 consensus sequences from GenBank sequences
NGF GGTGCATAGCGTAATGTCCA TTGCTCCTGTGAGTCCTGTT 55 372 GenBank XM_227525
TGF-β CGGCAGCTGTACATTGACTT AGCGCACGATCATGTTGGAC 60 271 GenBank AF000133
CathK AGCTGGGGAGAAAGCTGGGGAAACAAAG AGGCACAAACAAATGGGGAAACCAAACA 65 245 GenBank D14036
OPN CCGATGACTCTCACCACTCC CCTCTTCACTCTTCGGCTCG 60 549 GenBank D11411
TSP-1 AGTGACTCAGCAGATGATGG CACAGGTGACAGAGCAGATG 65 403 GenBank D16544
VEGF GGAGTACCCTGATGAGATCGA CTTTGGTCTGCATTCACATTTGT 60 211 GenBank AF022179
β-Actin TGCTTCTAGGCGGACTGTTA CGTCACATGGCATCTCACGA 55 314 GenBank U07786

The indicated forward and reverse primers for the molecules indicated in Lane 1 were validated for use in the rabbit. The size of the expected PCR product for each molecule is given in base pairs (Lane 5). The original sources of information used to develop the indicated primer sets is given in Lane 6. Several of the indicated primer sets have been used in previous rabbit studies [20,27].

Achari, Mol Vis 2008; 14:443-455. http://www.molvis.org/molvis/v14/a54

©2008 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535