Table 2 of Polisetty, Mol Vis 2008; 14:431-442.

Table 2. Population doublings of cultured MC-L from P0 through P6.

Passage number Initial cell density Incubation time (Days) Mean final cell number (x millions) Number of cell doublings (NCD) Accumulative NCD Population doubling time (h)
P0 23
P1 3–4 1.8
P2 1×104 10 3.0 8.2295 8.2295 29.1633
P3 1×104 10 1.3 7.0230 15.2525 34.1734
P4 1×104 10 0.13 3.7008 18.9533 64.8508
P5 1×104 10 0.08 3.0002 21.9535 79.9946
P6 1×104 10 0.02 1.000 22.9535 240

The table summarizes the results of population doubling study of cultured MC-L from passages 0 (P0) to 6 (P6), indicating the initial cell density, mean final cell number and the number of cell doublings calculated using the formula. Passages P0 and P1 have not been included for calculations due to epithelial cell contamination.

Polisetty, Mol Vis 2008; 14:431-442. http://www.molvis.org/molvis/v14/a53

©2008 Molecular Vision http://www.molvis.org/molvis/

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