Molecular Vision: Yan, Mol Vis 2008; 14:418-424. Table 1

Table 1. Primers used to screen mutations.

Gene symbol and accession number	Sequence	Amplified fragment	Annealing temperature*	Size
*GJA8* GI:6942063	F:CGGGGCCTTCTTTGTTCTCTAGTCC R:AGGCCCAGGTGGCTCAACTCC	The first part of exonic fragment	60	877 bp
*GJA8* GI:6942063	F:CAGCCGGTGGCCCTGCC R:GTTGCCTGGAGTGCACTGCCC	The second part of exonic fragment	69	907 bp
*CRYGC* GI: 37551287	F:TCAATCATATAGACAGAGCCA R:ATGTCCATCTAACCCTTAGGT	Exons 1 and 2	55	784 bp
*CRYGC* GI: 37551287	F:AATGACAATTCCATGCCACA R:CCCACCCCATTCACTTCTTA	Exon 3	55	534 bp
*CRYGD* GI: 37551287	F:TGATAGCAATCCGAATACTCCA R:GGGTAATACTTTGCTTATGTGGGGAG	Exons 1 and 2	55	776 bp
*CRYGD* GI: 37551287	F:GTCCTCACCAAGCTGGACTG R:CCATTTGCCTCGTGTGTGTA	Exon 3	55	496 bp
*CRYBB2* GI: 16168698	F:CACTGTGTCCAAGGTCACACAGCTAAGC R:CCCCTCGTTCACCCTCCCATCA	Exon 6	69	506 bp
*CRYAA* GI: 37559293	F:CTCCAGGTCCCCGTGGTA R:AGGAGAGGCCAGCACCAC	Exon 1	60	251 bp
	F:CTGTCTCTGCCAACCCCAG R:CTGTCCCACCTCTCAGTGCC	Exon 2	65	220 bp
	F:GGCAGCTTCTCTGGCATG R:GAGCCAGCCGAGGCAATG	Exon 3	60	309 bp
*CRYAB* GI: 27540935	F:AGGATGAATTACCCGGACAGAAAG R:ACCCCTGATCCCGACTGTTAT	Exon 2	65	360 bp
*CRYAB* GI: 27540935	F:TGAGTTCTGGGCAGGTGATAATAGTT R:AGCTTGATAATTTGGGCCTGCC	Exon 3	60	391 bp

F: forward primer, R: reverse primer. The genomic DNA was amplified in a 25 μl reaction volume with 10 pmol forward primers and reverse primers. The asterisk (*) indicates that the PCR conditions were the following: denaturation at 95 °C for 5 min followed by 35 cycles of denaturation at 95 °C for 45 s, annealing at the Annealing Temp for 30 s, and extension at 72 °C for 30 s with the last extension for 10 min at 72 °C.