Figure 8. Standard immunofluorescence staining on Chang (WKD) and IOBA-NHC cells. Phalloidin and propidium iodide stainings show the morphology of Chang cells (A,B) and IOBA-NHC cells (C,D) after 15 min of treatment with solutions of PBS (A,C) and BAC 10⁻²% (B,D). Nuclei were stained in red by propidium iodide and cytoskeleton (F-actin) in green by phalloidin. Note the BAC-dependent increase of cell shrinkage on both cell lines. BAC, benzalkonium chloride; PBS, phosphate buffered saline.