Figure 7. Unbound VEGF. A: Retinas from 50/10 oxygen-induced retinopathy (OIR) noninjected pups at p14 were incubated overnight in either 1 µg neutralizing antibody vascular endothelial growth factor (VEGFab) or IgG. Incubation with VEGFab reduced detectable free vascular endothelial growth factor (VEGF) compared to control IgG. B: Eyes in 50/10 OIR injected with 50 ng VEGFab at p12 showed significantly increased free VEGF in the retina at p13 compared to those injected with IgG (n=5). Asterisk (*) indicates p=0.042 based on Student’s t-test.