Molecular Vision: Wood, Mol Vis 2008; 14:334-344. Table 1

Table 1. Effect of different light intensities on serum-deprivation or rotenone-induced cell viability decrease in cultured RGC-5 cells.

Treatment	% viability (compared to dark plus serum control)	n
Dark + serum	100	10
Dark – serum	83.48±56*	10
1000 lux light + serum	82.87±1.78**	10
1000 lux light – serum	66.59±3.03***	10
4000 lux light + serum	77.81±4.19**	4
4000 lux light – serum	†53.66±4.51***	4
100 pM rotenone + dark	88.48±1.33*	8
100 pM rotenone + 1000 lux light	68.90±6.12**	8
+ 10 mM trolox	95.33±4.64‡	4
100 pM rotenone + 4000 lux light	56.22±4.10 **	4

Culture viability was determined by assaying cells for their ability to reduce the dye, 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to an insoluble blue formazan product after treatments for 48 h. The various treatments were performed as defined in the table; rotenone was applied in the presence of serum. The data clearly show that the increased light exposure levels (4000 lux) had a greater effect on exacerbation of cell damage caused by either serum-deprivation or rotenone than the lower light level (1000 lux). Data are shown as mean ± SEM and related as a percentage of dark + serum control and statistical significance denoted by *p <0.05, ** p <0.01, or ***p <0.001, when a one-way ANOVA followed by a post-hoc Bonferroni test was performed, and when when comparing with controls incubated in the dark for 48 h in the presence of 10% (v/v) serum. Further significance is denoted as follows: †p<0.05, when comparing indicated samples with cells exposed to 1000 lux light in the absence of serum; ‡p<0.05, when comparing indicated samples with cells exposed to 100 pM rotenone and 1000 lux light in the presence of serum.