Figure 5. Confocal images of AQP4 labeling in the retina after 48 hours of occlusion. Composite confocal micrograph of a transverse section of the retina after 48 h of occluder wear is displayed. The left section demonstrates the differential interference contrast image and the right section illustrates the AQP4 immunofluorescent image in red. The middle section shows both images superimposed. Note that AQP4 labeling is predominant in the nerve fiber, ganglion cell and inner plexiform layers of the inner retina, just as demonstrated by the normal eye (see Figure 1). However, the intensity of labeling is greater along the vitreal border in the form-deprived eye than in a normal eye. Bar is 50 μm.